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Antigenic Specificity | CD154, Human |
Clone | 5C8 |
Host Species | Mouse |
Reactive Species | human, nonhuman primate |
Isotype | IgG2aκ |
Format | APC-Vio 770 conjugate |
Size | 30 tests in 60 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | CD154 Antibody, anti-human, APC-Vio® 770. The antibody specifically recognizes the human CD154 antigen, a 39 kDa transmembrane glycoprotein, also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in T cell/antigen-presenting cell interactions through ligation of CD40. The antibody has been shown to block the activation of antigen-presenting cells by T helper cells in vitro. Because of its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells. The addition of a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154 expression induced by interaction with CD40 expressed on antigen-presenting cells. For intracellular detection of CD154 expression or if a pure population of enriched T cells is used, blocking of CD40 is not required. |
Immunogen | n/a |
Other Names | CD40LG, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39 |
Gene, Accession # | Gene ID: 959 |
Catalog # | 130-114-130 |
Price | $158 |
Order / More Info | CD154, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Frentsch, M. et al. (2005) Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat Med 11: 1118-1124. |