Edit |   |
---|---|
Antigenic Specificity | eNOS, Human |
Clone | REA451 |
Host Species | Recombinant Human |
Reactive Species | human, mouse |
Isotype | IgG1 |
Format | allophycocyanin (APC) conjugate |
Size | 30 tests in 300 µL |
Concentration | 1:11 |
Applications | Intracellular flow cytometry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | eNOS Antibody, anti-human, APC, REAfinity™. Clone REA451 recognizes the human endothelial nitric oxide synthase (eNOS) antigen regardless of phosphorylation status. eNOS catalyzes the production of nitric oxide (NO) from l-arginine within the endothelium to regulate vascular tone via cGMP signaling in vascular smooth muscle, mitochondrial respiration, platelet function, inflammation, and angiogenesis. Physiologically, the most important stimulus for the continuous formation of NO is the shear stress generated by the streaming blood on the endothelial layer. Serine/threonine protein kinase Akt/PKB mediates the activation of eNOS, leading to increased NO production. Inhibition of the phosphatidylinositol-3-OH kinase/Akt pathway or mutation of the Akt site on eNOS protein attenuates the serine phosphorylation and prevents the activation of eNOS. | Additional information: Clone REA451 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | NOS3, ECNOS, NOS type III, EC-NOS, NOS III |
Gene, Accession # | Gene ID: 4846 |
Catalog # | 130-106-840 |
Price | $140 |
Order / More Info | eNOS, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Dimmeler, S. et al. (1999) Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature 399 (6736): 601-605. | King, A. L. et al. (2014) Hydrogen sulfide cytoprotective signaling is endothelial nitric oxide synthase-nitric oxide dependent. Proc. Natl. Acad. Sci. U.S.A. 111 (8): 3182-3187. | Janssens, S. P. et al. (1992) Cloning and expression of a cDNA encoding human endothelium-derived relaxing factor/nitric oxide synthase. J. Biol. Chem. 267 (21): 14519-14522. |