IL-4, Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG
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| Antigenic Specificity | IL-4, Mouse |
| Clone | BVD4-1D11 |
| Host Species | Rat |
| Reactive Species | mouse |
| Isotype | IgG2bκ |
| Format | Vio B515 conjugate |
| Size | 30 µg in 1 mL |
| Concentration | 1:10 |
| Applications | Intracellular flow cytometry |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | IL-4 Antibody, anti-mouse, Vio® B515. Interleukin-4 (IL-4) is predominantly secreted by activated CD4+ memory and effector T?2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T?2 types of immune mechanisms can also be involved in immunological disorders such as IgE-mediated allergy. | The Anti-IL?4 antibody has been designed for intracellular staining of IL?4-producing cells. Cells can be stimulated for IL?4-production, e.g., by polyclonal stimulation with mitogens. For induction of IL?4 production by antigen-specific T cells, cells are restimulated with respective antigen. IL?4 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL?4-producing cells can be stained intracellularly with the Anti-IL?4 antibody. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL?4-producing cells. Magnetically enriched cells can be stained intracellularly for IL?4 production directly on the MACS® Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures for cytokine analysis of rare cells, e.g., CD4+ T?cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages, or synovial fluids. |
| Immunogen | n/a |
| Other Names | BSF-1 |
| Gene, Accession # | Gene ID: 16189 |
| Catalog # | 130-108-167 |
| Price | $178 |
| Order / More Info | IL-4, Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG |
| Product Specific References | n/a |
MILTENYI BIOTEC B.V. & Co. KG
MILTENYI BIOTEC B.V. & Co. KG
Friedrich-Ebert-StraÃe 68
51429 Bergisch Gladbach GERMANY
P: +49 2204 8306-0
F: +49 2204 85197
macs@miltenyibiotec.de
https://www.miltenyibiotec.com
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