Edit |   |
---|---|
Antigenic Specificity | Ly-6G, Mouse |
Clone | REA526 |
Host Species | Recombinant Human |
Reactive Species | mouse |
Isotype | IgG1 |
Format | allophycocyanin (APC) conjugate |
Size | 30 µg in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Ly-6G Antibody, anti-mouse, APC, REAfinity™. Clone REA526 recognizes the mouse lymphocyte antigen 6G (Ly-6G) antigen, a 21-25 kDa glycosylphosphatidylinositol (GPI)-linked protein which is highly expressed on neutrophilic granulocytes. Expression greatly increases during granulocyte maturation. To a lower extent, Ly-6G is also expressed during the development of monocytes. In mouse bone marrow samples, Ly-6G is primarily detected on granulocytes, but not on lymphocytes or erythrocytes. Ligation of Ly-6G in animals with an underlying background inflammation (induced by TNF-α) leads to profound cardiovascular and respiratory responses, including severe breathing difficulties, leukocyte and platelet aggregation, and coagulation, leading to microvessel obstruction and subsequent death. | Additional information: Clone REA526 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | Ly-6G.1, Ly-6G.1, Ly6g, Gr-1 |
Gene, Accession # | Gene ID: 546644 |
Catalog # | 130-120-803 |
Price | $93 |
Order / More Info | Ly-6G, Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J Immunol 150 (12): 5379-5390. | Carr, K. D. et al. (2011) Specific depletion reveals a novel role for neutrophil-mediated protection in the liver during Listeria monocytogenes infection. Eur. J. Immunol. 41 (9): 2666-2676. | Tanaka, Y. et al. (2012) Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J. Leukoc. Biol. 91 (3): 485-494. |