IL-5, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG
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| Antigenic Specificity | IL-5, Human |
| Clone | JES1-39D10 |
| Host Species | Rat |
| Reactive Species | human, nonhuman primate |
| Isotype | IgG2aκ |
| Format | Vio B515 conjugate |
| Size | 100 tests in 200 µL |
| Concentration | 1:50 |
| Applications | Intracellular flow cytometry |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | IL-5 Antibody, anti-human, Vio® B515. Interleukin 5 (IL-5) is pre-dominantly secreted by CD4+ T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells. | The Anti-IL-5 antibody has been designed for intracellular staining of IL-5-producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5-producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5-producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS® Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4+ T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids. |
| Immunogen | n/a |
| Other Names | EDF, TRF |
| Gene, Accession # | Gene ID: 3567 |
| Catalog # | 130-127-976 |
| Price | $355 |
| Order / More Info | IL-5, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
| Product Specific References | n/a |
MILTENYI BIOTEC B.V. & Co. KG
MILTENYI BIOTEC B.V. & Co. KG
Friedrich-Ebert-StraÃe 68
51429 Bergisch Gladbach GERMANY
P: +49 2204 8306-0
F: +49 2204 85197
macs@miltenyibiotec.de
https://www.miltenyibiotec.com
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