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Antigenic Specificity | MHC Class II (I-Ab), Mouse |
Clone | REA528 |
Host Species | Recombinant Human |
Reactive Species | mouse |
Isotype | IgG1 |
Format | APC-Vio 770 conjugate |
Size | 30 µg in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | MHC Class II (I-Ab) Antibody, anti-mouse, APC-Vio® 770, REAfinity™. Clone REA528 recognizes the mouse MHC class II alloantigen I-Ab of H-2b bearing mouse strains. It also cross-reacts with the H-2k and H-2u haplotypes, but reactivity to other haplotypes like, for example, d, f, g7, p, q, r, and s has not been observed. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow. | Additional information: Clone REA528 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | MHC class II IAb |
Gene, Accession # | Gene ID: 14961 |
Catalog # | 130-120-299 |
Price | $460 |
Order / More Info | MHC Class II (I-Ab), Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Cuenca, M. et al. (2016) Targeting of Ly9 (CD229) disrupts marginal zone and B1 B cell homeostasis and antibody responses. J Immunol 196: 726-737. | Gradehandt, G. et al. (1993) The I-Ab-restricted alloresponse of D10.G4.1 T cells is based on the recognition of an endogenous peptide. Immunology 78 (4): 592-599. | Tobita, T. et al. (2003) A role for the P1 anchor residue in the thermal stability of MHC class II molecule I-Ab. Immunol. Lett. 85 (1): 47-52. | Zhu, Y. et al. (2003) Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif. J. Mol. Biol. 326 (4): 1157-1174. |