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Antigenic Specificity | CD20 Cytoplasmic, Human |
Clone | REA1087 |
Host Species | Recombinant Human |
Reactive Species | human, mouse |
Isotype | IgG1 |
Format | phycoerythrin (PE) conjugate |
Size | 200 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | CD20 Cytoplasmic Antibody, anti-human, PE, REAfinity™. Clone REA1087 recognizes an intracytoplasmic epitope localized on the CD20 antigen, which is a non-glycosylated transmembrane protein of 33-37 kDa that is expressed on B lineage cells from the pre-B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca2+ channel and might function in the regulation of local responses during B cell activation. In vitro effects of CD20-specific antibodies on resting B cells indicate that CD20 is able to transduce an extracellular signal affecting the G0/G1 cell cycle transition. Studies have demonstrated that CD20-initiated intracellular signals involve tyrosine kinase activation and that CD20 is tightly associated with both serine and tyrosine kinases. | Additional information: Clone REA1087 displays negligible binding to Fc receptors. |
Immunogen | n/a |
Other Names | CD20cy, CD20 cytoplasmic domain |
Gene, Accession # | Gene ID: 931, 12482 |
Catalog # | 130-118-293 |
Price | $315 |
Order / More Info | CD20 Cytoplasmic, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Einfeld, D. A. et al. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. EMBO J. 7 (3): 711-717. | Deans, J. P. et al. (1995) Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J. Biol. Chem. 270 (38): 22632-22638. | Vaughan, A. T. et al. (2015) Activatory and inhibitory Fcγ receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain. J. Biol. Chem. 290 (9): 5424-5437. |