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Antigenic Specificity | Vimentin |
Clone | [RV202] |
Host Species | Mouse |
Reactive Species | dog, chicken, Caprine, hamster, human, monkey, mouse, rat, porcine, zebrafish |
Isotype | IgG1 |
Format | unconjugated |
Size | 0.1 mg |
Concentration | n/a |
Applications | Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP. |
Immunogen | Source Note: RV202 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal vimentin extract of calf lens. |
Other Names | [vimentin; Vimentin; vimentin; OTTHUMP00000019224; OTTHUMP00000019225; vimentin], [VIM; VIM; FLJ36605] |
Gene, Accession # | Gene ID: 7431, NCBI: NP_003371.2, UniProt: P08670 |
Catalog # | MBS570132 |
Price | $490 |
Order / More Info | Vimentin Antibody from MYBIOSOURCE INC. |
Product Specific References | Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of keRatin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Viebahn, C., Lane, E. B., and Ramaekers, F. C. (1988). KeRatin and vimentin expression in early organogenesis of the Rabbit embryo, Cell Tissue Res 253, 553-62. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. |