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Antigenic Specificity | His-Tag |
Clone | [11E12] |
Host Species | Mouse |
Reactive Species | General |
Isotype | IgG1 kappa |
Format | Protein A purified |
Size | 0.1 mL |
Concentration | 1 mg/ml |
Applications | Western Blot (WB), ELISA (EIA) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Monoclonal Antibody to detect His-taaged proteins. Background: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest. |
Immunogen | Immunogen: 6X-HIS-KLH. Positive Control: Western blot: His-Tagged protein |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS840047 |
Price | $375 |
Order / More Info | His-Tag Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |