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Antigenic Specificity | GFP |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | unconjugated |
Size | 0.05 mL, 0.1 mL, 0.2 mL |
Concentration | n/a |
Applications | Western Blot (WB), Immunoprecipitation (IP) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: GFP Antibody detects GFP and GFP fusion proteins. The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing exp |
Immunogen | Source Note: This polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of EGFP. |
Other Names | n/a |
Gene, Accession # | [GFP], NCBI: ACY56286.1 |
Catalog # | MBS435032 |
Price | $215, $275, $370 |
Order / More Info | GFP Antibody from MYBIOSOURCE INC. |
Product Specific References | 1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805. |