Edit |   |
---|---|
Antigenic Specificity | N-WASP (Tyr-256), phospho-specific |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, rat, mouse |
Isotype | n/a |
Format | affinity purified |
Size | 0.1 mL |
Concentration | n/a |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier). The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine. Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin poly |
Immunogen | Immunogen: Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291. |
Other Names | [N-WASP (Tyr-256); phospho-specific; Neural Wiskott-Aldrich syndrome protein; WASL], [WASL; WASL; NWASP; N-WASP; MGC48327; DKFZp779G0847] |
Gene, Accession # | Gene ID: 8976, NCBI: NP_003932.3, UniProt: O00401 |
Catalog # | MBS472041 |
Price | $385 |
Order / More Info | N-WASP (Tyr-256), phospho-specific Antibody from MYBIOSOURCE INC. |
Product Specific References | • Baba, Y. et al. (1999) Blood 93:2003.• Torres, E. & Rosen, M.K. (2003) Mol Cell 11:1215.• Wu, X. et al. (2004) J Biol Chem 279(10):9565. |