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Antigenic Specificity | 6x-His Tag |
Clone | [2C5.1] |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG2a |
Format | Protein A purified |
Size | 0.1 mg |
Concentration | n/a |
Applications | Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags o |
Immunogen | Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells. |
Other Names | n/a |
Gene, Accession # | [6x-His Tag] |
Catalog # | MBS395186 |
Price | $340 |
Order / More Info | 6x-His Tag Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |