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Antigenic Specificity | His tag tag |
Clone | polyclonal |
Host Species | Mouse |
Reactive Species | All species |
Isotype | n/a |
Format | peroxidase (HRP) conjugate |
Size | 0.1 mg |
Concentration | n/a |
Applications | Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Detects over-expressed or recombinant proteins containing the 6 x His epitope tag. Background/Introduction: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study pro |
Immunogen | n/a |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS355032 |
Price | $250 |
Order / More Info | His tag tag Antibody from MYBIOSOURCE INC. |
Product Specific References | 1. Hengen, P (1995). Purification of His-Tag fusion proteins from Escherichia coli. Trends in Biochemical Sciences 20 (7): 285-6. 2. Gavin, AC; Bosche, M; Krause, R; Grandi, P; Marzioch, M; Bauer, A; Schultz, J; Rick, JM et al. (2002). Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415 (6868): 141-7. |