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Antigenic Specificity | Cyclin B1 (G2- & M-phase Cyclin) |
Clone | [CCNB1/1098] |
Host Species | Mouse |
Reactive Species | human, mouse |
Isotype | IgG1, kappa |
Format | unconjugated |
Size | 0.02 mg (With BSA & Azide at 0.2mg/ml), 0.1 mg (With BSA & Azide at 0.2mg/ml), 0.1 mg (Without BSA & Azide at 1mg/ml) |
Concentration | n/a |
Applications | Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays |
Immunogen | Immunogen: Recombinant human full-length CCNB1 protein |
Other Names | [CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1], [CCNB1; CCNB1; CCNB; CCNB] |
Gene, Accession # | [CCNB1], Gene ID: 891, NCBI: NP_114172.1, UniProt: P14635 |
Catalog # | MBS438517 |
Price | $190, $340, $340 |
Order / More Info | Cyclin B1 (G2- & M-phase Cyclin) Antibody from MYBIOSOURCE INC. |
Product Specific References | Galaktionov, K. and Beach D. 1991. Specific activation of Cdc25 tyrosine phosphatases by B type cyclins: Evidence for multiple roles of mitotic cyclins. Cell 67: 1181-1194 |