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Antigenic Specificity | CDC25C |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat |
Isotype | n/a |
Format | immunogen affinity purified |
Size | 0.1 mL |
Concentration | 1.0 mg/ml |
Applications | Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The antibody detects endogenous level of total CDC25C protein. Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). |
Immunogen | Immunogen Type: Recombinant Protein Immunogen Description: Recombinant protein of human CDC25C. |
Other Names | [CDC25; PPP1R60] |
Gene, Accession # | [CDC25C], Gene ID: 995, NCBI: NP_001274511.1, UniProt: P30307 |
Catalog # | MBS9412194 |
Price | $255 |
Order / More Info | CDC25C Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |