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Antigenic Specificity | BrdU |
Clone | monoclonal |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG1 |
Format | affinity purified |
Size | 0.1 mL |
Concentration | 1mg/ml |
Applications | Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may |
Immunogen | Immunogen: Bromodeoxyuridine |
Other Names | n/a |
Gene, Accession # | [BrdU] |
Catalog # | MBS126158 |
Price | $275 |
Order / More Info | BrdU Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |