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Antigenic Specificity | EAF1 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat |
Isotype | n/a |
Format | affinity purified |
Size | 0.1 mL |
Concentration | n/a |
Applications | Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interactio |
Immunogen | Immunogen: Fusion protein of human EAF1 Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Other Names | [ELL associated factor 1; EAF1] |
Gene, Accession # | [EAF1], Gene ID: 85403, NCBI: NP_149074.3, UniProt: Q96JC9 |
Catalog # | MBS8502108 |
Price | $310 |
Order / More Info | EAF1 Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |