actin alpha-smooth Antibody from MYBIOSOURCE INC.
Search, find, compare suppliers for anti-actin alpha-smooth antibody, protein, ELISA kits.
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| Antigenic Specificity | actin alpha-smooth |
| Clone | [1A4] |
| Host Species | Mouse |
| Reactive Species | chicken, Caprine, monkey, quail, rat, sheep, porcine, Xenopus |
| Isotype | IgG2a |
| Format | unconjugated |
| Size | 0.05 mg |
| Concentration | n/a |
| Applications | Electron microscopy, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB) |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Specificity: alpha-SM1 reacts exclusively with alpha-smooth muscle actin which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope that is recognized by alpha-SM1 is Ac-EEED. Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Alpha-smooth muscle actin is abundant in |
| Immunogen | Source Note: alpha-SM1 (clone 1A4) is a Mouse monoclonal IgG2a antibody derived by fusion of Sp2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the first 10 amino acids of alpha-smooth muscle actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin via the C-terminal cysteine (Ac-EEEDSTALVC). |
| Other Names | n/a |
| Gene, Accession # | n/a |
| Catalog # | MBS570033 |
| Price | $500 |
| Order / More Info | actin alpha-smooth Antibody from MYBIOSOURCE INC. |
| Product Specific References | Skalli, O., Ropraz, P., Trzeciak, A., Benzonana, G., Gillessen, D. and Gabbiani, G. (1986). A monoclonal antibody anti alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103, 2787-96. Skalli, O., Schurch, W., Seemayer, T., Lagace, R., Montandon, D., Pittet, B. and Gabbiani, G. (1989). Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. Lab Invest 60, 275-85. Babaev, V.R., Bobryshev, Y.V., Stenina, O.V., Tararak, E.M. and Gabani, G. (1990). Heterogeneity of smooth muscle in atheromatous plaque of Human aorta. American Journal of Pathlogy 136, 1031-42. Sappino, A. P., Schurch, W. and Gabbiani, G. (1990). Differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations. Lab Invest 63, 144-61. Vyalov, S. L., Gabbiani, G. and Kapanci, Y. (1993). Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis. Am J Pathol 143, 1754-65. Chaponnier, C., Goethals, M., Janmey, P. A., Gabbiani, F., Gabbiani, G. and Vandekerckhove, J. (1995). The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo. J Cell Biol 130, 887-95. Simoncelli, F., Fagotti, A., Di Rosa, I., Panara, F., Chaponnier, C., Gabbiani, G. and Pascolini, R., (1996). Expression of an actin in protochordates and lower craniates defined by anti-aSM-1. European J of Cell Biol 69, 297-300. Hinz, B., Celetta, G., Tomasek, J. J., Gabbiani, G. and Chaponnier, C. (2001). Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol Biol Cell 12, 2730-41. Hinz, B., Gabbiani, G. and Chaponnier, C. (2002). The NH2-terminal peptide of alpha-smooth muscle actin inhibits force geneRation by the myofibroblast in vitro and in vivo. J Cell Biol 157, 657-63. Hinz, B., Dugina, V., Ballestrem, C., Wehrle-Haller, B. and Chaponnier, C. (2003). Alpha-smooth muscle actin is crucial for focal adhesion matuRation in myofibroblasts. Mol Biol Cell 14, 2508-19. Clement, S., Hinz, B., Dugina, V., Gabbiani, G. and Chaponnier, C. (2004). The N-terminal Ac-EEED sequence plays a role in alpha-smooth-muscle actin incorpoRation into stress fibers. Journal of Cell Science 118, 1395-1404. Chaponnier, C. and Gabbiani, G. (2004). Pathological situation characterized by altered actin isoform expression. J Pathol 204, 386-95. Clement, S., Stouffs, M., Bettiol, E., Kampf, S., Krause, K., Chaponnier, C. and Jaconi, M. (2006). Expression and function of alpha-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation. Journal of Cell Science 120, 229-38. De Visscher, G., Plusquin, R., Mesure, L. and Flameng, W. (2010). Selection of an immunohistochemical panel for cardiovascular research in Sheep. Appl Immunohistochem Mol Morphol 18, 382-91. |
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