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Antigenic Specificity | HIS tag (H20) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | unconjugated |
Size | 0.2 mg |
Concentration | 0.200 mg/ml |
Applications | Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Detects proteins with the polyhistidine Tag. The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation. |
Immunogen | Immunogen: Synthetic a peptide containing the 6x histidine tag. |
Other Names | n/a |
Gene, Accession # | [HIS] |
Catalog # | MBS440057 |
Price | $240 |
Order / More Info | HIS tag (H20) Antibody from MYBIOSOURCE INC. |
Product Specific References | 1.) Wu B, Wilmouth RC. 2008. Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot. Mol Cells. Feb 29;25(1):43-9. |