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Antigenic Specificity | His-tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | immunogen affinity purified |
Size | 0.03 mL, 0.1 mL, 0.2 mL |
Concentration | 1 mg/ml |
Applications | Western Blot (WB), ELISA (EIA) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein's structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins. |
Immunogen | Immunogen: 6X His synthetic peptide conjugated to KLH |
Other Names | [Rabbit His-tag; His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag] |
Gene, Accession # | [HIS] |
Catalog # | MBS9700849 |
Price | $130, $220, $325 |
Order / More Info | His-tag Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |