Edit |   |
---|---|
Antigenic Specificity | EGFR (Ser-1142), phospho-specific |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, rat, mouse |
Isotype | n/a |
Format | affinity purified |
Size | 0.1 mL |
Concentration | n/a |
Applications | ELISA, Western Blot, Immunocytochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-1142) coupled to agarose then affinity purified using phospho-EGFR (Ser-1142) peptide (without carrier). The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment. The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGF |
Immunogen | Immunogen: Phospho-EGFR (Ser-1142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 1142 ofhuman EGFR (ErbB-1). This human EGFR sequence has high homology with rat and mouse EGFR, and is not conserved in otherErbB family members. |
Other Names | [Epidermal Growth Factor Receptor; ErbB-1], [EGFR; EGFR; ERBB; HER1; mENA; ERBB1; PIG61; ERBB1] |
Gene, Accession # | Gene ID: 1956, NCBI: NP_005219.2, UniProt: P00533 |
Catalog # | MBS472023 |
Price | $385 |
Order / More Info | EGFR (Ser-1142), phospho-specific Antibody from MYBIOSOURCE INC. |
Product Specific References | • Carpenter, G. (2000) Bioessays 22:697.• Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107. |