Edit |   |
---|---|
Antigenic Specificity | Maltose Binding Protein (MBP) Epitope Tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | purified |
Size | 1 mg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin. |
Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein. |
Other Names | rabbit anti-MBP Epitope Tag Antibody, rabbit anti-Maltose Binding Protein Antibody |
Gene, Accession # | n/a |
Catalog # | 200-401-385 |
Price | $525 |
Order / More Info | Maltose Binding Protein (MBP) Epitope Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 25756623 // 27182662 // 29358299 // 30089259 |