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Antigenic Specificity | BrdU, CldU, IdU |
Clone | 29G6.E8 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG1 lambda |
Format | Protein A purified |
Size | 100 µg |
Concentration | 1.0 mg/ml |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. |
Immunogen | Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development. |
Other Names | mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU |
Gene, Accession # | n/a |
Catalog # | 200-301-H50 |
Price | $485 |
Order / More Info | BrdU, CldU, IdU Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 28122984 // 29474907 // 35923694 |