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Antigenic Specificity | Avidin |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | fluorescein (FITC) conjugate |
Size | 25 µL |
Concentration | 1.0 mg/mL |
Applications | FLISA, Flow Cytometry, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-Avidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Avidin. Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. |
Immunogen | Avidin (Hen Egg White) |
Other Names | rabbit anti-Avidin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Avidin antibody, Avidin fluorescein, Anti-Avidin FITC Antibody, egg ehite |
Gene, Accession # | UniProt: P02701 |
Catalog # | 200-4297S |
Price | $125 |
Order / More Info | Avidin Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |