Edit |   |
---|---|
Antigenic Specificity | IdU |
Clone | 32D8.D9 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG1 kappa |
Format | Protein A purified |
Size | 100 µg |
Concentration | 1.0 mg/ml |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU. Cross-reactivity is not observed with BrdU, CldU or FdU. Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. |
Immunogen | Anti-IdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development. |
Other Names | mouse anti-IdU antibody, Iododeoxyuridine, 5-iodo-2'-deoxyuridine |
Gene, Accession # | n/a |
Catalog # | 200-301-H51 |
Price | $485 |
Order / More Info | IdU Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 31331069 |