Collagen Type II Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | Collagen Type II |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | human, bovine |
| Isotype | n/a |
| Format | immunoaffinity purified |
| Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Applications | ELISA, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of `type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research. |
| Immunogen | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
| Other Names | rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody |
| Gene, Accession # | COL2A1, Gene ID: 1280, NCBI: NP_001835.3, UniProt: P02458 |
| Catalog # | 600-401-104-0.1 |
| Price | $485 |
| Order / More Info | Collagen Type II Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 17298979 // 17785842 // 19660048 // 23107560 // 24056368 // 25061805 // 25160583 // 27885831 // 28084417 // 28273419 // 29346562 // 29624608 // 30125096 // 30559329 // 30862915 // 31534979 // 31536840 // 31932796 // 31994552 // 32011382 // 32078578 // 32223949 // 32578533 // 33465893 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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