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Antigenic Specificity | NOTCH 1 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse |
Isotype | n/a |
Format | serum |
Size | 25 µL |
Concentration | 80 mg/mL |
Applications | ELISA, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Anti Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). |
Other Names | rabbit anti-notch1 antibody, Neurogenic locus Notch homolog protein 1, hN1, Translocation-associated Notch protein TAN-1 |
Gene, Accession # | NOTCH1, Gene ID: 4851, NCBI: NP_060087.3, UniProt: P46531 |
Catalog # | 100-401-407S |
Price | $125 |
Order / More Info | NOTCH 1 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 18334253 // 19828677 // 23248117 // 23497505 // 24201805 // 26253400 // 27913625 // 32235870 |