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Antigenic Specificity | AKT phospho T308 |
Clone | 18F3.H11 |
Host Species | Mouse |
Reactive Species | human, mouse, rat, monkey |
Isotype | IgG1 kappa |
Format | DyLight™ 649 conjugate |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. |
Immunogen | This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein. |
Other Names | mouse anti-AKT pT308 DyLight™649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
Catalog # | 200-343-269 |
Price | $465 |
Order / More Info | AKT phospho T308 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |