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Antigenic Specificity | Collagen Type III |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, bovine, porcine |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.16 mg/mL |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of `type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research. |
Immunogen | Collagen Type III from human and bovine placenta |
Other Names | rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain |
Gene, Accession # | COL3A1, Gene ID: 1281, NCBI: NP_000081.1, UniProt: P02461 |
Catalog # | 600-401-105-0.1 |
Price | $485 |
Order / More Info | Collagen Type III Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 10864813 // 11021707 // 12231207 // 18332234 // 19262461 // 19339678 // 20305780 // 20516667 // 20570839 // 20804746 // 20888336 // 21543445 // 22008391 // 22052862 // 22164265 // 23027345 // 23398466 // 23673415 // 23781260 // 24140724 // 24218554 // 24374669 // 24529431 // 24655808 // 25196145 // 25685931 // 25892322 // 26749086 // 26808140 // 26905437 // 27265328 // 27466370 // 27754873 // 28453726 // 28860484 // 28887614 // 29351412 // 29351434 // 29895208 // 30388216 // 31100613 // 31415839 // 32409493 // 32764293 // 32768595 // 33124967 // 33184116 |