IL-1 Beta Antibody from ROCKLAND IMMUNOCHEMICALS INC.

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Antigenic SpecificityIL-1 Beta
Clonepolyclonal
Host SpeciesRabbit
Reactive Specieshuman
Isotypen/a
Formatserum, no preservative
Size1 mg
Concentration2.0 mg/mL
ApplicationsELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
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DescriptionThis is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW human IL-1β and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1β in the same formats using similar dilutions. The antiserum does not recognize human IL-1α. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1β containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1β precursor molecule. Denatured 31,000 precursor IL-1β will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1β exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1β precursor in lysates. IL-1 beta (also known as Interleukin-1 beta, IL-1β and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1β is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
ImmunogenThis antibody was prepared by repeated immunizations with recombinant human IL-1β produced in E.coli. The MW of the recombinant 153 aa IL-1β was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1β converting enzyme (ICE, capase-1).
Other Namesrabbit anti-IL-1 beta Antibody, rabbit anti-interleukin-1 beta antibody, IL-1 beta, Interleukin-1 beta, IL-1β, catabolin
Gene, Accession #IL1B, Gene ID: 3553, NCBI: P01584.2, UniProt: P01584
Catalog #209-401-301
Price$1225
Order / More InfoIL-1 Beta Antibody from ROCKLAND IMMUNOCHEMICALS INC.
Product Specific ReferencesPubMed: 27273825 // 31428213
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654

orders@rockland.com
Technical Support: tech@rockland.com

https://www.rockland.com/

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