RFP, mCherry, rRFP Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | RFP, mCherry, rRFP |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | n/a |
| Isotype | n/a |
| Format | immunoaffinity purified |
| Size | 25 µL |
| Concentration | 1.04 mg/mL |
| Applications | ELISA, Immunohistochemistry, IF Microscopy, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal. mCherry antibody is ideal for western blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. |
| Immunogen | The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
| Other Names | rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein |
| Gene, Accession # | DsRed |
| Catalog # | 600-401-P16S |
| Price | $125 |
| Order / More Info | RFP, mCherry, rRFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 24027267 // 26208961 // 26770887 // 27086548 // 28358926 // 28366204 // 28858616 // 29709033 // 30635240 // 31582560 // 31719175 // 31811775 // 32166324 // 32632196 // 33006316 // 33369805 // 33525646 // 34595172 // 34643468 // 34962957 // 35013168 // 35077502 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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