6X His Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | 6X His Tag |
| Clone | 33D10.D2.G8 |
| Host Species | Mouse |
| Reactive Species | n/a |
| Isotype | IgG1 kappa |
| Format | peroxidase (HRP) conjugate |
| Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Applications | ELISA, Immunohistochemistry, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | 6X His antibody is directed against the 6X His motif and was purified from concentrated tissue culture supernate by Protein A chromatography. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag and is useful in determining its presence in various assays. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin. |
| Immunogen | This 6X his antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
| Other Names | mouse anti-6X His Tag HRP conjugated Antibody, peroxidase conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody |
| Gene, Accession # | n/a |
| Catalog # | 200-303-382 |
| Price | $455 |
| Order / More Info | 6X His Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 28300475 // 31695028 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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