DYKDDDDK (FLAG® tag) Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Edit | |
|---|---|
| Antigenic Specificity | DYKDDDDK (FLAG® tag) |
| Clone | 29E4.G7 |
| Host Species | Mouse |
| Reactive Species | n/a |
| Isotype | IgG2a kappa |
| Format | DyLight™ 649 conjugate |
| Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Applications | ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
| Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. |
| Other Names | mouse anti-FLAG™ tag DyLight™649 conjugation, Enterokinase Cleavage Site (ECS), DyLight™649 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
| Gene, Accession # | n/a |
| Catalog # | 200-343-383 |
| Price | $425 |
| Order / More Info | DYKDDDDK (FLAG® tag) Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | n/a |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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