FLAG™ Antibody from ROCKLAND IMMUNOCHEMICALS INC.
Search, find, compare suppliers for anti-FLAG™ antibody, protein, ELISA kits.
| Edit | |
|---|---|
| Antigenic Specificity | FLAG™ |
| Clone | 29E4.G7 |
| Host Species | Mouse |
| Reactive Species | n/a |
| Isotype | IgG2a kappa |
| Format | Protein A purified |
| Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
| Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. |
| Other Names | mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
| Gene, Accession # | n/a |
| Catalog # | 200-301-383 |
| Price | $279 |
| Order / More Info | FLAG™ Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 25739390 // 25767741 // 26966178 // 27485701 // 28264028 // 28700677 // 32286293 // 32886017 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
Profile of ROCKLAND IMMUNOCHEMICALS INC.
Return to Antibodies