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Antigenic Specificity | RFP, rRFP, tdTomato |
Clone | polyclonal |
Host Species | Chicken |
Reactive Species | n/a |
Isotype | n/a |
Format | unconjugated |
Size | 25 µL |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot. Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP. |
Immunogen | Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
Other Names | chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583 |
Gene, Accession # | DsRed UniProt: Q9U6Y8 |
Catalog # | 600-901-379S |
Price | $125 |
Order / More Info | RFP, rRFP, tdTomato Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 25629932 // 25897521 // 27153332 // 27427984 // 27527793 // 27731315 // 28498105 // 28506464 // 28817806 // 28942923 // 29489752 // 29891732 // 29937280 // 30146301 // 30154505 // 30282023 // 30451684 // 30551997 // 30901089 // 31363138 // 31439641 // 31493814 // 31515492 // 31613218 // 31640762 // 31696318 // 31974446 // 32210746 // 32243809 // 32378798 // 32516597 // 32541005 // 32562755 // 32582852 // 32634398 // 32639590 // 32826903 // 33046884 // 33221554 // 33370772 // 33589589 // 33927593 // 33960573 // 34171291 // 34242703 // 34330268 // 34509687 // 34693906 // 34851821 // 35196485 |