Beta Actin Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | Beta Actin |
| Clone | 3H2.E4.E12 |
| Host Species | Mouse |
| Reactive Species | human, mouse |
| Isotype | IgG2a lambda |
| Format | Protein A purified |
| Size | 25 µL |
| Concentration | 1.0 mg/mL |
| Applications | ELISA, Immunohistochemistry, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse β Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. |
| Immunogen | Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with β-Actin peptide near the N-Terminus followed by hybridoma development. |
| Other Names | mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, β actin, anti-beta actin antibody |
| Gene, Accession # | ACTB, Gene ID: 11461, NCBI: NP_001092.1, UniProt: P60709 |
| Catalog # | 200-301-E69S |
| Price | $125 |
| Order / More Info | Beta Actin Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 24410055 // 27191820 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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