CDC27 phospho S426 Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | CDC27 phospho S426 |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | human, rat, chicken, chimpanzee, dog |
| Isotype | n/a |
| Format | immunoaffinity purified |
| Size | 100 µg |
| Concentration | .99 mg/mL |
| Applications | ELISA, Immunoprecipitation, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pS426 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CDC27 pS426 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, chicken and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse may occur as sequence homology varies by one amino acid residue in this sequence (89% homology). Cross reactivity with CDC27 homologues from other sources has not been determined. Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of Human cdc27 amino acids 415-445 surrounding S426. |
| Other Names | rabbit anti-CDC27 pS426 Antibody, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3 |
| Gene, Accession # | CDC27, D0S1430E, Gene ID: 996, NCBI: 167466175, UniProt: P30260 |
| Catalog # | 600-401-866 |
| Price | $475 |
| Order / More Info | CDC27 phospho S426 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | n/a |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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