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Antigenic Specificity | DYKDDDDK (FLAG® tag) |
Clone | 29E4.G7 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG2a kappa |
Format | agarose conjugate |
Size | 1 mL |
Concentration | 1.0 mg |
Applications | Immunoprecipitation |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG® epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) is recommended for competitive elution to recover fusion protein (see protocol). Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG® and HA. |
Immunogen | Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH. |
Other Names | mouse anti-FLAG® tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody |
Gene, Accession # | n/a |
Catalog # | 200-350-383 |
Price | $535 |
Order / More Info | DYKDDDDK (FLAG® tag) Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |