Protein L Ligand Leakage ELISA XL from ANIARA
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| Product Name | Protein L Ligand Leakage ELISA XL |
| Description | Medicago AB Products Are Available Worldwide. For Research Use Only. This Protein L Ligand Leakage ELISA Kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, the samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a colour change which is proportional to the amount of bound Protein L from the sample. The colour change is quantified spectrophotometrically at 450 nm by using a plate reader. The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e. g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. |
| Size | 1 kit |
| Concentration | n/a |
| Applications | n/a |
| Other Names | n/a |
| Gene, Accession, CAS # | n/a |
| Catalog # | A10-0028-1 |
| Price | $2305 |
| Order / More Info | Protein L Ligand Leakage ELISA XL from ANIARA |
| Product Specific References | n/a |
ANIARA
ANIARA
7768 Service Center Dr.
West Chester OH 45069
P: 513-770-1991
P: 866-783-3797
F: 513-573-9241
http://www.aniara.com
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