Spark Blue™ 488 FLAER from BIOLEGEND

The bacterial toxin aerolysin has the remarkable ability to bind selectively and with high affinity to the glycophosphatidylinositol (GPI) anchors of cell surface proteins, causing lysis of normal but not GPI-deficient cells. FLAER is a fluorescently-labeled, inactive variant of aerolysin that does not cause lysis of cells. The reagent binds selectively to cell surface GPI-anchored proteins, but will not bind to cells that are missing GPI-anchors, such as cells with deficiency in any of the phosphatidylinositol glycan complementation groups (PIG) genes.