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Product Name | HL-60 Whole Cell Lysate |
Description | HL-60 Whole Cell Lysate. HL-60 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/ml with 1X RIPA buffer. |
Size | 500 ug |
Concentration | 4.0 mg/ml |
Applications | ChIP, IP, SDS-PAGE, WB |
Other Names | HL-60 Lysate, Cell Lysate, HL60 lysate |
Gene, Accession, CAS # | n/a |
Catalog # | orb348702 |
Price | $289 |
Order / More Info | HL-60 Whole Cell Lysate from BIORBYT LTD. |
Product Specific References | n/a |
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