Edit |   |
---|---|
Product Name | Rat L-FABP ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the FABP present in samples reacts with the anti-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FABP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FABP in the test sample. The quantity of FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 3.125 ng/ml - 200 ng/ml. Sensitivity: 1.278 ng/ml. Incubation time: 80 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | L-FABP test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | LFP21-K01 |
Price | $600 |
Order / More Info | Rat L-FABP ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |