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Product Name | SeroCT C. trachomatis IgG ELISA Assay Kit |
Description | he Eagle Biosciences SeroCT plates are coated with C. trachomatis specific peptides. Serum to be tested is diluted and incubated with the precoated SeroCT plate 1h at 37°C. In this step C. trachomatis specific antibodies are bound to the immobilized C. trachomatis specific peptides. Non specific antibodies are removed by washing. Anti-human IgG conjugated to horseradish peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized peptides. Sample types: serum. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 2.25 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | C. trachomatis IgG, SeroCT test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 181-01 |
Price | $450 |
Order / More Info | SeroCT C. trachomatis IgG ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |