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Product Name | Human nFGF-21 ELISA Assay Kit |
Description | This Eagle Biosciences Human N-terminal FGF-21 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human N-terminal FGF-21 in serum and EDTA-plasma sample. The assay utilizes the two-site ``sandwich'' technique with two selected antibodies that bind to different epitopes of human FGF-21. One of the antibodies specifically binds to the N-terminal human FGF-21 (1-7) and the other is to the multi-epitopes of mid-regional and C-terminal human FGF-21. Assay calibrators, controls and patient samples are added directly to wells of a microplate that is coated with an anti-human FGF-21 (1-7) specific antibody. After the first incubation period, a horseradish peroxidase-conjugated anti-human FGF-21 polyclonal antibody is added to each well. After the second incubation period, the antibody on the wall of microtiter well captures human N-terminal FGF-21 in the sample and further forms ``sandwich'' with the tracer antibody. Unbound proteins in each microtiter well are washed away. An immunocomplex of ``anti-FGF-21 antibody --- human N-terminal FGF-21 --- HRP-conjugated tracer antibody'' is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human N-terminal FGF-21 on the wall of the microtiter well is directly proportional to the amount of N-terminal FGF-21 in the sample. A calibrator curve is generated by plotting the absorbance versus the respective human intact FGF-21 concentration for each calibrator on point-to-point or 4 parameter curve fit. The concentration of human N-terminal FGF-21 in test samples is determined directly from this calibrator curve. Sample types: serum, EDTA plasma. Range: 54 - 2100 pg/mL. Sensitivity: 4.7 pg/mL. Incubation time: 2.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | nFGF-21. Fibroblast Growth Factor 21, FGF-21 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | N2131-K01 |
Price | $715 |
Order / More Info | Human nFGF-21 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |