Angiopoietin-2 ELISA from EAGLE BIOSCIENCES INC.
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| Product Name | Angiopoietin-2 ELISA |
| Description | The Angiopoietin-2 human ELISA kit is a sandwich enzyme immunoassay for the quantitative determinationof angiopoietin-2 in human serum and plasma samples. Standards, controls and samples must be pre-diluted 1+10 prior to assaying. In a first step, pre-diluted standard/control/sample and biotinylated antibody (goat polyclonal anti-human angiopoietin-2) are pipetted into the wells of the microtiter strips, which are pre-coated with a monoclonal anti-human angiopoietin-2 antibody. Angiopoietin-2 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the anti-human angiopoietin-2 antibody. In a washing step all non-specific unbound material is removed. In a second step, the conjugate (Streptavidin-HRP) is pipetted into the wells and reacts with the biotinylated antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of angiopoietin-2 present in the sample. This color change is detectable with a standard microplate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) using the values obtained from the standards versus the standard concentration is generated. The concentration of angiopoietin-2 in the sample is determined directly from the dose response curve. Sample types: serum, EDTA plasma, citrate plasma, heparin plasma. Range: 0 - 400 pmol/l. Sensitivity: 3.7 pmol/l. Incubation time: 3.5 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Angiopoietin-2, ANG-2 test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | BI-ANG2 |
| Price | $545 |
| Order / More Info | Angiopoietin-2 ELISA from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.