Triiodothyronine - Free (fT3) ELISA from EAGLE BIOSCIENCES INC.
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| Product Name | Triiodothyronine - Free (fT3) ELISA |
| Description | The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of fT3 in the sample. A set of standards is used to plot a standard curve from which the amount of fT3 in patient samples and controls can be directly read. The labelled T3 (conjugate) employed in this assay system has shown no substantial binding properties towards thyroxine-binding globulin (TBG) or human serum albumin (HSA). The binding sites on the microplates are designed to be of a low binding-capacity in order not to disturb the equilibrium between T3 and its carrying proteins. The assay is carried out under normal physiological conditions of pH, temperature and ionic strength. Sample types: serum. Range: 2-40 pg/mL. Sensitivity: 0.3 pg/mL. Incubation time: 75 minutes |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Triiodothyronine test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | T3F31-K01 |
| Price | $215 |
| Order / More Info | Triiodothyronine - Free (fT3) ELISA from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.