Edit |   |
---|---|
Product Name | Vitamin K1 HPLC Assay |
Description | For the determination of vitamin K1 an internal standard is added first. After a solid phase extraction on SPE-cartridges, the samples are precipitated. The supernatant is then extracted with an organic solvent and evaporated. After that the samples are re-suspended and injected into the HPLC system. A post-column reduction reactor reduces vitamin K and enables the measurement of vitamin K with a fluorescence detector. The isocratic separation at 30°C using a ``reverse phase'' column lasts 20 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed by the delivered plasma calibrator; the concentration is calculated by the internal standard method via integration of the peak areas resp. heights. Sample types: serum, plasma. Incubation time: 10 minutes |
Size | 100 tests |
Concentration | n/a |
Applications | RUO |
Other Names | Vitamin K, phylloquinone, Vitamin K1 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | VK131-H100 |
Price | $900 |
Order / More Info | Vitamin K1 HPLC Assay from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |