Total Glutathione Assay Kit from EAGLE BIOSCIENCES INC.
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| Product Name | Total Glutathione Assay Kit |
| Description | The Total Glutathione (tGSH) Microplate Assay kit employs a kinetic enzymatic recycling assay based on the oxidation of GSH by 5,5'-dithiobis(2-nitrobenzoic acid) [DTNB] to measure the total glutathione (tGSH) content of biological samples. The included glutathione standards or treated samples are added to the microtiter plate wells, followed by DTNB and glutathione reductase. Addition of b-NADPH2 to the wells initiates the progressive reduction of DTNB by GSH, causing a color increase that is monitored at 405 nm. The rate of color change, followed over a 5 minute time period, is proportional to the tGSH concentration. Consequently, the concentration of tGSH in unknown samples may be determined by reference to the standard curve. GSH reacts with DTNB to produce a colored ion, which absorbs light at 405 nm, and a mixed disulphide. This disulphide reacts with further quantities of GSH present to liberate another ion and GSSG. GSSG is reduced enzymatically to GSH which then re-enters the cycle (see figure below). Since GSSG represents only a small percentage of total acid-solution free glutathione, the resulting values for tGSH (which encompasses both GSH and GSSG) are expressed in units of GSH equivalents. Sample types: biological fluids. Range: 0.5 - 20 µM. Sensitivity: 0.5 µM. Incubation time: 20 minutes |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Glutathione, GSH, TGSH, Total Glutathione, test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | GLU39-K01 |
| Price | $435 |
| Order / More Info | Total Glutathione Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.