C5a, Human, ELISA kit, 2 x 96 det. from EAGLE BIOSCIENCES INC.

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Product NameC5a, Human, ELISA kit, 2 x 96 det.
DescriptionThe human C5a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 31/2 hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C5a. Biotinylated tracer antibody will bind to the captured human C5a. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C5a standards (log). The human C5a concentration of samples, which are run concurrently with the standards, can be determined from the standard curve. Sample types: serum, plasma. Range: 0.3 to 20 ng/ml. Sensitivity: 0.3 ng/ml. Incubation time: 3.5 hours
Size2 x 96 well
Concentrationn/a
ApplicationsRUO
Other Nameskit, assay, EIA, enzyme immunoassay
Gene, Accession, CAS #n/a
Catalog #HC531-K02
Price$1485
Order / More InfoC5a, Human, ELISA kit, 2 x 96 det. from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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