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Product Name | Secretin (Human) (Chirhotim) ELISA |
Description | The Human Secretin ELISA is a competitive immunoassay for the determination of Human Secretin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Human Secretin bound. Sample types: serum, plasma and tissue extracts. Range:.01-1000 ng/ml. Incubation time: minimum 1 hour 30 minutes |
Size | 96 wells |
Concentration | n/a |
Applications | RUO |
Other Names | Secretin test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | KBI5027 |
Price | $1020 |
Order / More Info | Secretin (Human) (Chirhotim) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |